全文获取类型
收费全文 | 5512篇 |
免费 | 525篇 |
国内免费 | 5篇 |
出版年
2023年 | 29篇 |
2022年 | 18篇 |
2021年 | 131篇 |
2020年 | 87篇 |
2019年 | 108篇 |
2018年 | 148篇 |
2017年 | 119篇 |
2016年 | 214篇 |
2015年 | 292篇 |
2014年 | 343篇 |
2013年 | 372篇 |
2012年 | 515篇 |
2011年 | 509篇 |
2010年 | 299篇 |
2009年 | 263篇 |
2008年 | 363篇 |
2007年 | 359篇 |
2006年 | 326篇 |
2005年 | 258篇 |
2004年 | 277篇 |
2003年 | 217篇 |
2002年 | 238篇 |
2001年 | 58篇 |
2000年 | 54篇 |
1999年 | 45篇 |
1998年 | 59篇 |
1997年 | 33篇 |
1996年 | 23篇 |
1995年 | 29篇 |
1994年 | 24篇 |
1993年 | 17篇 |
1992年 | 24篇 |
1991年 | 20篇 |
1990年 | 20篇 |
1989年 | 17篇 |
1988年 | 10篇 |
1987年 | 11篇 |
1986年 | 10篇 |
1985年 | 15篇 |
1984年 | 19篇 |
1983年 | 5篇 |
1982年 | 12篇 |
1981年 | 10篇 |
1980年 | 7篇 |
1979年 | 9篇 |
1978年 | 5篇 |
1977年 | 3篇 |
1974年 | 4篇 |
1971年 | 4篇 |
1968年 | 2篇 |
排序方式: 共有6042条查询结果,搜索用时 0 毫秒
11.
12.
13.
14.
The century-old idea that stripes make zebras cryptic to large carnivores has never been examined systematically. We evaluated this hypothesis by passing digital images of zebras through species-specific spatial and colour filters to simulate their appearance for the visual systems of zebras’ primary predators and zebras themselves. We also measured stripe widths and luminance contrast to estimate the maximum distances from which lions, spotted hyaenas, and zebras can resolve stripes. We found that beyond ca. 50 m (daylight) and 30 m (twilight) zebra stripes are difficult for the estimated visual systems of large carnivores to resolve, but not humans. On moonless nights, stripes are difficult for all species to resolve beyond ca. 9 m. In open treeless habitats where zebras spend most time, zebras are as clearly identified by the lion visual system as are similar-sized ungulates, suggesting that stripes cannot confer crypsis by disrupting the zebra’s outline. Stripes confer a minor advantage over solid pelage in masking body shape in woodlands, but the effect is stronger for humans than for predators. Zebras appear to be less able than humans to resolve stripes although they are better than their chief predators. In conclusion, compared to the uniform pelage of other sympatric herbivores it appears highly unlikely that stripes are a form of anti-predator camouflage. 相似文献
15.
Silvane M. F. Murta Tim J. Vickers David A. Scott Stephen M. Beverley 《Molecular microbiology》2009,71(6):1386-1401
10-Formyl tetrahydrofolate (10-CHO-THF) is a key metabolite in C1 carbon metabolism, arising through the action of formate-tetrahydrofolate ligase (FTL) and/or 5,10-methenyltetrahydrofolate cyclohydrolase/5,10-methylene tetrahydrofolate dehydrogenase (DHCH). Leishmania major possesses single DHCH1 and FTL genes encoding exclusively cytosolic proteins, unlike other organisms where isoforms occur in the mitochondrion as well. Recombinant DHCH1 showed typical NADP+ -dependent methylene tetrahydrofolate DH and 5,10-methenyltetrahydrofolate CH activities, and the DH activity was potently inhibited by a substrate analogue 5,10-CO-THF ( K i 105 nM), as was Leishmania growth (EC50 1.1 μM). Previous studies showed null ftl - mutants were normal, raising the possibility that loss of the purine synthetic pathway had rendered 10-CHO-THF dispensable in evolution. We were unable to generate dhch1 - null mutants by gene replacement, despite using a wide spectrum of nutritional supplements expected to bypass DHCH function. We applied an improved method for testing essential genes in Leishmania , based on segregational loss of episomal complementing genes rather than transfection; analysis of ∼1400 events without successful loss of DHCH1 again established its requirement. Lastly, we employed 'genetic metabolite complementation' using ectopically expressed FTL as an alternative source of 10-CHO-THF; now dhch1 - null parasites were readily obtained. These data establish a requirement for 10-CHO-THF metabolism in L. major , and provide genetic and pharmacological validation of DHCH as a target for chemotherapy, in this and potentially other protozoan parasites. 相似文献
16.
17.
Protein breakdown in pulse-labelled and longlabelled cells of Arthrobacter S
1-55, a psychrotrophic bacterium, has been assessed at different temperatures. The temperature at which the cells were grown and labelled affected the breakdown of pulsed-labelled but not long-labelled proteins. Inhibitors of ATP synthesis inhibited proteolysis. Miscoding antibiotics stimulated the production of rapidly degradable proteins. 相似文献
18.
Wayne W. Poon Anthony J. Carlos Brittany L. Aguilar Nicole C. Berchtold Crystal K. Kawano Vahe Zograbyan Tim Yaopruke Michael Shelanski Carl W. Cotman 《The Journal of biological chemistry》2013,288(23):16937-16948
We previously found that BDNF-dependent retrograde trafficking is impaired in AD transgenic mouse neurons. Utilizing a novel microfluidic culture chamber, we demonstrate that Aβ oligomers compromise BDNF-mediated retrograde transport by impairing endosomal vesicle velocities, resulting in impaired downstream signaling driven by BDNF/TrkB, including ERK5 activation, and CREB-dependent gene regulation. Our data suggest that a key mechanism mediating the deficit involves ubiquitin C-terminal hydrolase L1 (UCH-L1), a deubiquitinating enzyme that functions to regulate cellular ubiquitin. Aβ-induced deficits in BDNF trafficking and signaling are mimicked by LDN (an inhibitor of UCH-L1) and can be reversed by increasing cellular UCH-L1 levels, demonstrated here using a transducible TAT-UCH-L1 strategy. Finally, our data reveal that UCH-L1 mRNA levels are decreased in the hippocampi of AD brains. Taken together, our data implicate that UCH-L1 is important for regulating neurotrophin receptor sorting to signaling endosomes and supporting retrograde transport. Further, our results support the idea that in AD, Aβ may down-regulate UCH-L1 in the AD brain, which in turn impairs BDNF/TrkB-mediated retrograde signaling, compromising synaptic plasticity and neuronal survival. 相似文献
19.
20.
The α -toxin (phospholipase C) of Clostridium perfringens has been reported to contain catalytically essential zinc ions We report here that histidine residues are essential for the co-ordination of these ion(s). Incubation of alpha toxin with diethylpyrocarbonate, a histidine modifying reagent, did not result in the loss of phospholipase C activity unless the protein was first incubated with EDTA, suggesting that zinc ions normally protect the susceptible histidine residues. When the amino acid sequences of three phospholipase C's were aligned, essential zinc binding histidine residues in the non-toxic B. cereus phospholipase C were found in similar positions in the toxic C. perfringens enzyme and the weakly toxic C. bifermentans phospholipase C. 相似文献